Rob Roy had been due to play Cumnock at Guy's Meadow but the ground will now host Cumbernauld United's Scottish Junior Cup replay with Newtongrange Star, which was called off last week.
Instead Stewart Maxwell's side will travel south to play Super League newcomers Girvan.
Meanwhile Rossvale's match has also been changed because scheduled opponents East Kilbride Thistle have a rearranged Cup clash with Thorniewood United to play.
Vale will now travel to the Brig O'Lea Stadium to face Neilston.
Kick-offs on Saturday - weather permitting - will be at 1.45pm.
Meanwhile Rob Roy went down 1-0 away to Kelty Hearts in a friendly match on Tuesday.
Jordyn Sheerin got the only goal of the match for the Fife side five minutes from time. Both sides used the match to give some of their younger players a runout.